NIMP| SYSTEM FOR THE DELIVERY IN TIME OF BIOACTIVE COMPONENTS USED IN THE RESTORATION OF ENZYMATIC HOMEOSTASIS OF SKIN WOUNDS

SYSTEM FOR THE DELIVERY IN TIME OF BIOACTIVE COMPONENTS USED IN THE RESTORATION OF ENZYMATIC HOMEOSTASIS OF SKIN WOUNDS

Project Director: Dr. Petre BADICA

Title: System for the delivery in time of bioactive components used in the restoration of enzymatic homeostasis of skin wounds

Acronim: SYSDELBIO

Contract 63PED/2025

PN-IV-P7-7.1-PED-2024-2569

Partners:

Coordonator/Coordinator - National Institute of Materials Physics, NIMP (Institutul Național de Cercetare Dezvoltare pentru Fizica Materialelor, INCDFM) (www.infim.ro)
Partener/Partner - Biotehnos SA (https://biotehnos.ro/)

Project Abstract

The need to identify new therapeutic approaches in the management of skin wounds has initiated new R&D strategies that explore emerging technologies to achieve therapeutic solutions with an effective healing process. This project proposes the design and preparation of a delivery system (TRL 4) with time-release, being made of substrates (e.g. plastic foils and 3D printed porous bodies) loaded with polymeric coatings containing biocompatible, biodegradable, and bioactive ingredients. New combinations of active ingredients such as protease enzymes, MgB2 and plant extracts with a standardized phytocompound content (flavones, polyphenols and phenols) from the Asteraceae family are used in these systems. The proposed combination is expected to exhibit synergistic or cumulative healing effects, providing multiple functionalities, e.g. antimicrobial, anti-inflammatory, regenerative, balancing, analgesic, others. Depending on the sequence and release timing of the active components of the delivery system, they will act efficiently and in an adapted manner versus specifics and condition of the wound which can have a wide spectrum of injury and healing stages.

Project team

INCDFM

Petre Badica

Andrei Alexandru-Dinu

Mihai-Alexandru Grigoroscuta

Cristina Bartha

Alina Marinela Badea (Ionescu)

Any Cristina Sergentu

Andrei Kuncser

Claudiu Locovei

Nicolae Dan BATALU

Biotehnos S.A.

Bogdan Purcareanu

Laura Olariu

Manuela Diana Ene

Sabina Cristiana Serbu

Roxana Andreea Nita

Veronica Drumea

Alexandru Mihai Florea

Alina Perisoara

Luiza Mariana Tanase

Recent publications

C. Bartha, Claudiu Locovei, Andrei Alexandru-Dinu, Cezar Comanescu, Mihai Alexandru Grigoroscuta, Andrei Kuncser, Nicusor Iacob, Magda Galatanu, Aurel Leca, Petre Badica and Victor Kuncser, Cation distribution and its magnetic implications in gadolinium–iron garnets for an enhanced control of compensation temperature, Phys. Chem. Chem. Phys., 2025, 27, 22894-22900.

Main results

In 2025. The objectives of the stage were (i) the design and preparation of substrates and 3D wound healing systems, (ii) the selection of active principles based on physicochemical characterization and evaluation of biological effects at the cellular and acellular level and (iii) the manufacture of wound healing systems through coatings.

For the preparation of substrates and wound healing systems: (a) supports were designed and obtained. These were loaded with a biodegradable polymer that encapsulates active substances and desorption tests were performed; (b) Other supports studied and tested from the point of view of desorption of active substances are mesoporous materials; (c) Biodegradable layers (coatings) were manufactured that will be used to create the wound healing system.

For the selection of active substances (plant extracts, enzymes), tests were performed that targeted cell proliferation, tissue remodeling capacity and enzyme activity, as well as the determination of antioxidant potential by standardized methods (ABTS, DPPH, FRAP). The levels of peroxide and superoxide radicals were also analyzed, along with the activity of enzymes involved in phase I of the cellular response to oxidative stress. In parallel, DSC/TG studies coupled with mass spectroscopy (MS) were initiated aimed at increasing the stability of active substances sensitive to high temperatures or with increased volatility. Based on these results, the extracts were selected and loaded into the supports indicated above and their desorption profile was evaluated.

Cognitive and socio-economic impact

The project aims to obtain innovative solutions for the treatment of skin wounds with controllable effects in time and space. The active substances are extracted from plants and inorganic substances carefully selected to balance opposing effects or to obtain effective synergies in wound healing. In 2025: control of functional properties in garnets depends on internal substitutions and the investigation methodology in iron-based materials can be extended to different substances of interest in biomedical field including wound healing.

PROJECTS/ NATIONAL PROJECTS