Complex experimental and theoretical approaches in the evaluation of magnetic hyperthermia application.
Project Director: Dr. Gabriel SCHINTEIE
Beneficiary: Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii (UEFISCDI)
Proiect Code: PN-III-P1-1.1-TE-2021-1300
Contract no.: TE 91/16.05.2022
Project title: Complex experimental and theoretical approaches in the evaluation of magnetic hyperthermia applications.
Funding period: 13/05.2022- 12/05/2024
The contracted amount: 450.000 lei
Project director: Gabriel Schinteie
The project scope is to develop new experimental approaches for the evaluation of the most important parameters which influence the magnetic hyperthermia(MH), to provide original procedures/methodologies for the evaluation of the Specific Absorption Rate (SAR). New magnetic single-domain iron oxide-based nanoparticles for biomedical applications will be designed and in vitro MH assays will be approached. By using specific preparation procedures, suitable and functionalization of mono-dispersed spherical, cubic or acicular shaped nanoparticles, for tailoring their effective anisotropy constant will be synthesized. It is envisaged: (i) the systematic evaluation of the most important parameters of interest in hyperthermia, (ii) the evaluation of the relationships between the magnetic and morpho-structural parameters with impact on SAR and the determination of SAR in correlation to realistic volume fraction of nanoparticles dispersed in a media compatible with the human tissue, (iii) the development of original SAR evaluation procedures by accounting for the heat loses , (iv) the consideration the spatial distribution of the magnetic field and nanoparticle concentration as input data for the discrete estimation of the dissipated power and further inputs in the bio-heat transfer equation, (v) the optimization of the RF magnetic field exposures on the NP systems, in correlation to morpho-structural characteristics and volume fractions specific to MH applications.
The actual proposal promotes a complex study of systems of magnetic iron oxides nanoparticles, with various morphologies and covered by different surfactants which control the MNPs interactions. The main purpose of this project is to embed efficiently the results that will be obtained into a safer and reproductible method for better treatments against tumors. It is worth mention that in present the most commonly used treatments for tumors implies non-targeting methods that are highly toxic for both tumor cells and healthy cells. Thus, it is of paramount importance to achieve a low cost way for treatment of tumors in a localized manner to maintain the as much as possible the integrity of healthy cells. The novelty of the project resides in the extraction and the unitar evaluation of every morphology and of every magnetic parameter that infleunces the SAR in relation to system behavior versus the particle density. The aim is a detailed understanding of the methods to control the phenomena associated to each parameter involved in the hyperthermia processes as well as of the advancing protocols, offering support,consistency and trust in the future approaches in the domain.
Synthesis of at least 5 sets of samples for hyperthermia; Design and implementation of an adiabatically isolated device for compounds storage during exposure to RF fields for minimaize the heat loss; Original methods in the assessment of SAR distributions; In vitro testing of the hyperthermia efficency; All the results will be disseminated in high impact factor journals( minimum 4 publicationes) and communications at international/national conferences. A web-page with the most important results and the latest news in the field of project will be elaborated.
All the objectives provided in the implementation plan related to Stage 1 ("(“Processing and morpho-structural characterization of Fe based magnetic nanoparticles and their optimization in relation to different applications”) consisting of: i) Synthesis, morpho-structural and magnetic characterization of at least 5 systems of magnetic nanoparticles (objectives O1 and O2), ii) scientific research report, iii) 1 scientific article sent for publication in ISI listed journals, iv) creation of a website for project and v) participation in a national/international conference) were fulfilled integral.
Following the studies from specific literature, the preparation methods and precursors for obtaining magnetic monodomain particles, with specific morphologies, controlled size and a relatively narrow particle size distribution, were established.
Preliminary preparations of oxo-hydroxides and iron oxides (magnetite) were carried out by: i) co-precipitation, ii) treatment of acicular oxy-hydroxide precursors, iii) hydrothermal decomposition and iv) thermal decomposition of organo-metallic compounds in solvents with high boiling point.
The influence of the preparation conditions (molar ratio of surfactant mixture, time and temperature of thermal treatment) on the morphology and size of magnetite nanoparticles obtained by the thermal decomposition of organo-metallic compounds was studied. The correlation between the magnetite morphology and the mixture of surfactants was established.
Various systems of hydrophobic magnetite, magnetic monodomain, with spherical, hexagonal, cubic or polyhedral morphologies were prepared.
Starting from the hydrophobic particles synthesized by the thermal decomposition of organo-metallic compounds in solvents with a high boiling point, hydrophilic particles functionalized with azelaic acid were obtained .
Through morpho-structural measurements the crystalline phases, shape and size of the constituent nanoparticles were identified (iron oxide nanoparticles with diameters between 20-50 nm were processed).
Mössbauer spectroscopy measurements and magnetic measurements confirmed the formation of magnetic monodomain nanoparticles, that do not reach the superparamagnetic regime, but which have saturation magnetizations and coercive appropriate for magnetic hyperthermia applications.
The results obtained were disseminated through a participation in a national conference, 2 articles published in ISI rated journals and an article sent for publication to Materials magazine.
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