BIONMEM – Ionophore embedded membranes for ion sensing in biological fluids
Project Director: Dr. Anca ALDEA
Project Code: PN-III-P1-1.1-PD-2021-0319
Project Director: Dr. Anca Aldea
Project Type: National
Project framework program: P1 - Development of the national CD system - Human resources - Postdoctoral research projects (PD)
Funded by: Executive Unit for Financing Higher Education, Research, Development and Innovation, UEFISCDI
Contractor: National Research and Development Institute for Materials Physics
Status: In progress
Start date: 01.04.2022
End Date: 31.03.2024
The goal of this project is the development of bioinspired membranes for the incorporation of ionophores able to specifically detect relevant ions. These membranes will be immobilized at electrodes surface for the development of bio-analytical devices for real-time monitoring of biomarker electrolytes in biological media by electrochemical methods. The detection of sodium, potassium and chloride ions in sweat is focused, yet the possibility to extend the working principle to their detection in other relevant biological media or to detection of some other electrolytes is envisioned. Sodium, potassium and chloride are proposed due to their frequent involvement in a large range of biochemical process. Immobilization in bio-inspired membranes form bilipidic, liposomes or hydrogel layers ensures the functional conformation/structure of the ionophore and, on the other hand the increased biocompatibility of the device at epidermal level.
The flexibility of these sensors will be achieved by using electrospun polymeric fibers that ensure the nanostructuring of the electrode surface and a significant increase of its electroactive area, but also of its sensitivity. Studies to determine the influence of sweat interferences on the sensor response and biocompatibility in epidermal cell cultures will be performed. Nonetheless, the adaptability of this type of membranes will be demonstrated by applications of the device for detection of ions in serum and cellular culture media.
The main goal of this project is the development of ionphore-embedded membranes with application in bio-analytical devices for electrochemical detection of biomarker electrolytes in sweat but also in other biological media such as serum or cellular cultures.
The focus is the detection of sodium, potassium and chloride ions. In order to achieve this goal, the following objectives are envisaged:
O1: Membrane synthesis and ionophores incorporation. Bilipidic membranes, hydrogels and liposomes will be synthesized and the incorporation of ionophores investigated.
O2: Immobilization of the ionophore embedded membrane at the electrode surface. Interaction of the membrane with solid surface may lead to conformational/structural modifications and finding the optimal condition is relevant for achieving the best sensitivity and the lowest detection limit. The sensor performance will be investigated in artificial sweat but also on a biological model designed to detect short and transient responses to the sensor.
Director Proiect: Dr. Anca Aldea (U-1800-055N-8239)
Mentor Proiect: Prof. Ileana Cornelia Farcasanu (U-1700-033C-8247)
In the second stage of Project PD67 the main objective was to test the devices obtained for the detection and quantification of sodium, potassium and chlorine ions, as well as to determine the characteristics of the sensors. During this phase, the activities of obtaining electrodes from polycaprolactone electrospun polymer fibers and silicon/silicon dioxide waffers metallized with gold, the synthesis of membranes, the incorporation of the ionophore into the synthesized membranes, the immobilization of the membranes with the ionophore incorporated on the surface of the electrodes, continued.
Briefly, polycaprolactone electrospun polymeric fibers were obtained which were coated with a gold layer and electrodes obtained on silicon/silicon dioxide wafers metallized with an intermediate layer of titanium and gold. 3 types of membranes were synthesized: lipid membrane, hydrogel and liposomes. Ionophore incorporation was performed both before and after membrane synthesis. In order to characterize the surfaces obtained, they were analyzed electrochemically (cyclic voltammetry), morphologically (scanning electron microscopy) and structurally (infrared spectroscopy). Sensors obtained by immobilizing membranes with incorporated ionophore on surface were tested for the detection and quantification of sodium, potassium and chlorine ions. Biocompatibility tests were also carried out by using them as a substrate for the proliferation of cell cultures.
As a result of these activities, a 100% fulfillment of objective 1 (O1: Synthesis of the membrane and incorporation of ionophores) and 75% of objective 2 (O2: Immobilization of the membrane with ionophore embedded on the electrode surface) was achieved. Bilipid membranes, hydrogels and liposomes were synthesized and the ionophore incorporation method was optimized, gold surfaces modified with different membranes and ionophores were obtained and characterized, the membrane immobilization method on the electrode surface was optimized and the sensors were tested in order to establish their response to specific ions of the incorporated ionophores.
Anca ALDEA (DIBLĂ)
National Institute of Materials Physics
Laboratory of Multifunctional Materials and Structures
405A Atomistilor Street
077125, Magurele, Romania
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