Exploring the Alzheimer disease biomarkers: fabrication of new functional biomaterials and development of early diagnosis biosensor


Project Director: Dr. Enache Adrian

Project ID: PN-III-P4-ID-PCE-2020-1403

Project Director: Adrian Enache

Project type: National

Program: Proiecte de cercetare exploratorie

Founding: Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii, UEFISCDI

Contractor: Institutul National C-D pentru Fizica Materialelor

Status: In desfasurare

Begin: 4 ianuarie, 2021

End: 28 decembrie, 2023

The goal of this research project is to contribute/boost the RDI activities in areas of general social interest, to increase the capacity of the public research sector, and to respond to the global challenges affecting the Romanian/EU economy by implementing scientific research in the areas of Analytical chemistry, Healthcare, Biophysics and Biochemistry through exploration of Alzheimer disease biomarkers. The project address to the development of (i) new functional biomaterials based on functionalization of the nanostructured materials with Amyloid beta peptides and (ii) an electrochemical biosensor for evaluation of peptide Methionine sulfoxide reductase A enzyme activity, an enzyme related with early detection of Alzheimer disease. Several nanostructured materials will be fabricated and their surfaces will be modified with amyloid beta peptide, especially the sequences of 40 or 42 amino acids. These new surfaces will be tested, in order to evaluate the bioactivity properties, against neuronal cells. The electrochemical biosensor for monitoring, quantification and enzymatic activity evaluation of the Methionine sulfoxide reductase A will be developed at the surface modified dispensable screen printed carbon electrodes. The successful implementation of the project is expected to generate methodologies for new advanced biomaterials and will support the development of a new generation of functional biomaterials and biosensors that could be translated to industrial scale.

Dr. Adrian Enache
Dr. Madalina Maria Barsan
Dr. Victor Diculescu
Dr. Monica Enculescu
Dr. Alexandru Evangelidis
Dr. Mihaela Bacalum
Drd. Ricardo Leote
Drd. Melania Onea

Scientific Report Stage 1

During this stage, according to the work plan, five research activities were carried out with the objectives (i) the manufacture and characterization of nanostructured materials for the development of surfaces modified with amyloid peptides, (ii) the development of protocols for the preparation of amyloid solutions. at various stages of aggregation, (iii) functionalization of nanostructured surfaces with amyloid peptides to grow neuronal cells on these surfaces, (iv) electrochemical evaluation of the sensing element to develop a biosensor to determine methionine sulfoxide reductase (MsrA) activity; and (v) Manufacture of catalytic nanostructures necessary for the development of the transducer for the biosensor dedicated to the determination of MsrA activity. Thus, commercial polymeric membranes have been characterized, metallized and tested in cell cultures. Protocols have also been developed for the preparation of Abeta40 amyloid solutions at various stages of aggregation and morphologically modified surfaces with these peptides have been evaluated. At the same time, synthetic peptides containing methionine were electrochemically characterized and metallic electrophilated polymeric fibers and zinc oxide nanostructures were manufactured in order to develop a biosensor to determine the activity of MsrA enzyme.
All the proposed objectives were achieved and the results obtained were disseminated in three international conferences and a scientific article was sent for publication in the journal Scientific Reports.

Articles submitted for publication:

  1. Melania Onea, Mihaela Bacalum, Andreea Luminita Radulescu, Mina Raileanu, Liviu Craciun, Tiberiu Relu Esanu, Teodor Adrian Enache, Electrochemical evaluation of irradiated cells: the effect of proton beam on the B16 line culture, Scientific Reports

 

Conferences:

  1. A. Enache, M.L. Onea, M. Bacalum, Cells Under Irradiation – Electrochemical Evaluation, XXVIth International Symposium on Bioelectrochemistry and Bioenergetics of the Bioelectrochemical Society, 10.05.21 – 15.05.21, Cluj, Romania
  2. Leote, C. Sanz, A. Aldea, M.M. Barsan, V.C. Diculescu, Flexible Bio(sensors) for Point-of-Care Biomedical Applications, 72nd Annual Meeting of the International Society of Electrochemistry, 29.08.21 – 03.09.21, Jeju, Coreea de Sud
  3. L. Onea, T.A. Enache, E. Matei, M. Enculescu, M.I. Enculescu, Bio-Sensing properties of Single ZnO nanowire Field Effect Transistor, 72nd Annual Meeting of the International Society of Electrochemistry, 29.08.21 – 03.09.21, Jeju, Coreea de Sud

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