Publications

Novel complexes of M2LCl4 center dot nH(2)O type (M:Ni, n = 4; M:Cu, n = 3 and M:Zn, n = 0; L: ligand resulted from 1,4-phenylenediamine, 3,6-diazaoctane-1,8-diamine and formaldehyde one-pot condensation) were synthesized and characterised by microanalytical, ESI-MS, IR, UV-Vis, H-1 NMR and EPR spectra, magnetic data at room temperature and molar conductivities as well. The electrochemical behaviour of complexes was investigated by cyclic voltammetry. Simultaneous TG/DTA measurements were performed in order to evidence the thermal behaviour of the obtained complexes. Processes such as water elimination, fragmentation and oxidative degradation of the organic ligand as well as chloride elimination occurred during thermal decomposition. The antimicrobial assays demonstrate that the compounds exhibited good antibacterial activity, especially against S. aureus and E. coli strains, the most active being the copper(II) complex, which also exhibited the most prominent anti-biofilm effect, suggesting its potential use for the development of new antimicrobial agents. The biological activity was correlated with log P (ow) values. All complexes disrupt the membrane integrity of HCT 8 tumour cells.

Boron carbide ceramic was prepared by reactive Spark Plasma Sintering under N-2-atmosphere and for different heating times and maximum pressure regimes. Split-Hopkinson Pressure Bar (SHPB), indentation, XRD and microscopy measurements were performed for samples characterization. It is shown that SHPB toughness control depending on SPS regime is possible and the main reason is introduction of nitrogen into B4C ceramic. Complex relationships between processing conditions, sintering mechanism, material's specifics, static and dynamic mechanical properties are discussed. Improvement of dynamic toughness is through mechanisms resembling those working for static load conditions such as cracks deflection and pull out, but there are also significant differences. (c) 2013 Elsevier Ltd and Techna Group S.r.l. All rights reserved.

The objective of this study was to obtain graphene oxide starting from graphite and to covalently functionalize this nanomaterial with Cisplatin. The presence of the drug was pointed out using different methods like FTIR Spectroscopy, X-Ray Photoelectron Spectroscopy (XPS), Thermogravimetric analysis (TGA), RAMAN Spectroscopy, X-Ray Diffraction and Scanning electron microscopy (SEM).

Silicon substrates were irradiated at normal incidence with a femtosecond Ti:sapphire laser (Quatronix, 90 fs pulse duration, 1 kHz repetition rate, M-2 similar to 1.2, maximum energy peak 350 mJ) operating at a wavelength of 400 nm and focused via a microscope objective (Newport; UV Objective Model, 37x 0.11 N.A.). The laser scanning was assisted by liquids precursors media such as methanol and 1,1,2-trichlorotrifluoroethane. By altering the processing parameters, such as incident laser energy, scanning speed, and different irradiation media, various surface structures were produced on areas with 1 mm 2 dimensions. We analyzed the dependence of the surface morphology on laser pulse energy, scanning speed and irradiation media. Well ordered areas are developed without imposing any boundary conditions for the capillary waves that coarsens the ripple pattern. To assess biomaterial-driven cell adhesion response we investigated actin filaments organization and cell morphological changes following growth onto processed silicon substrates. Our study of bone cell progenitor interaction with laser nanoprocessed silicon lines has shown that cells anchor mainly to contact points along the nanostructured surface. Consequently, actin filaments are stretched towards the 15 mu m wide parallel lines increasing lateral cell spreading and changing the bipolar shape of mesenchymal stem cells.

New complexes ML(CNS)center dot nH(2)O [M = Ni, n = 0.5; M = Cu, n = 4.5; M = Zn, n = 0.5, HL: 6-mercapto-(1,4,8,11-tetraazaundecanyl)-6-carboxylic acid)] have been synthesised, chemical analysed, and characterised by different spectroscopic techniques (IR, UV-Vis-NIR, H-1 NMR, EPR, ESI-MS), and magnetic measurements. Based on the IR spectra a dinuclear structure with the 1,3-CSN coordination was proposed for Ni(II) and Cu(II) complexes. The dinuclear structure of Cu(II) complex is also consistent with both magnetic behaviour and EPR spectrum. According to TG, DTG and DTA curves the thermal transformations are complex processes, including dehydration, Mannich base oxidative degradation and thiocyanate decomposition. The final product of decomposition is the most stable metallic oxide, as XRD data indicates. The new complexes were also screened for their microbicidal and antibiofilm properties.

By performing comparative Raman studies on nanometric thin films (9.5, 39, 88 and 185 nm) of copper phthalocyanine (CuPc) deposited on glass, Au and Ag supports, we demonstrate that the mechanism of the surface-enhanced Raman scattering (SERS) generated on Au and Ag substrates differs in the Stokes and anti-Stokes Raman branches depending on whether non-resonant (515.5 nm) or resonant (647.1 nm) optical excitation is applied. The evaluation of the SERS effect via the I-aS/I-S ratio reveals that this ratio is smaller or larger than that predicted by the Boltzmann law for non-resonant or resonant optical excitation, respectively. In the former case, the enhancement of the Stokes Raman emission is similar to a stimulated Raman process resulting from the plasmon coupling associated with the incident excitation light and spontaneous Stokes Raman emission. For the latter case, the amplification of the anti-Stokes Raman emission results from a wave-mixing process reminiscent of a single-beam CARS effect.

We investigated the magnetic relaxation of weakly interacting, dispersive, single domain Fe nanoparticles in Fe/Al2O3 composites prepared by sequential pulsed laser deposition on (100) Si substrates. The external magnetic field was oriented parallel to the substrate and applied in zero-field cooling conditions. It was found that the relaxation of the irreversible magnetic moment m (irr) is similar to the well-known vortex-creep process in disordered superconductors with poor supercurrent redistribution across the sample, leading to a power-law dependence of m (irr)(t), with a well-defined time t exponent.

Hydroxyapatite (HA) is a widely used biomaterial for implant thin films, largely recognized for its excellent capability to chemically bond to hard tissue inducing the osteogenesis without immune response from human tissues. Nowadays, intense research efforts are focused on development of antimicrobial HA doped thin films. In particular, HA doped with Ag (Ag:HA) is expected to inhibit the attachment of microbes and contamination of metallic implant surface. We herewith report on nano-sized HA and Ag:HA thin films synthesized by pulsed laser deposition on pure Ti and Ti modified with 100 nm diameter TiO2 nanotubes (fabricated by anodization of Ti plates) substrates. The HA-based thin films were characterized by SEM, AFM, EDS, FUR, and XRD. The cytotoxic activity was tested with HEp2 cells against controls. The antifungal efficiency of the deposited layers was tested against the Candida albicans and Aspergillus niger strains. The Ti substrates modified with TiO2 nanotubes covered with Ag:HA thin films showed the highest antifungal activity. (C) 2013 Elsevier B.V. All rights reserved.

Bioactive glasses are osteoproductive-type inorganic materials possessing the highest indices of bioactivity in both bulk and thin film forms. The prerequisites for reliable implant-type coatings are both their biological and mechanical performances. Whilst the bioglass films' structural, chemical and biological properties have been studied extensively, information about their mechanical performance is scarce. Here, transmission electron microscopy, energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, nanoindentation and pull-out measurements were employed to assess the morphological, chemical, structural and mechanical properties of the bioglass films deposited onto Ti substrates by radio-frequency magnetron sputtering (RF-MS). The biological safety of the thin bioglass films was evaluated preliminarily in vitro by investigating the adherence, proliferation and cytotoxicity of fibroblast cells cultivated on their surface. Our study emphasize the versatility of RF-MS, showing how bioglass films' features such as composition, structure, bonding strength, hardness, elastic modulus and biological response can be conveniently adapted by tuning the RF-MS working conditions, and therefore demonstrating the unexplored potential of this deposition technique for preparing quality biomimetic glass coatings. (C) 2013 Elsevier B. V. All rights reserved.