GENERAL SEMINAR: Prof. Dr. habil. Fred Lisdat, Biosystems Technology, Institute of Life Sciences and Biomedical Technologies, Technical University of Applied Sciences Wildau, 15745 Wildau, Germany
ABOUT PROF. LISDAT
Fred Lisdat has studied chemistry at Humboldt University and got his phd from the same university in 1992. His research interest turned from chemical sensors towards biosensors and thus, he made is post doc at Kyushu University in Japan. Since 1994 he joined the research group of Prof. Scheller at Potsdam University. He finalised the habilitation there in 2004 on the „Sensorial detection of signal molecules using redox conversions at protein molecules”. 2001 he was working as guest professor at Tokyo University in Japan. 2004 he got the Professorship of Biosystems Technology at the Technical University of Wildau. 2016 he has been working as guest professor at the Tokyo University of Agriculture and Technology.
His research interests include biosensors, recycling systems, reactive oxygen species, protein electrochemistry, label-free detection of nucleic acids and proteins, artificial protein arrangements on electrodes, protein engineering, semiconductor nanostructures and conducting polymers, biofuel cells and photobioelectrochemistry.
Fred Lisdat has been active in several national and international organisations, e.g. he was the president of the regional BioHyTec association (2000-2021), chair/chair elect of the Bioelectrochemical Division of the International Society of Electrochemistry ISE (2015-2018), head group of the „Arbeitskreis Chemo -und Biosensorik” of the GdCh (Society of German Chemists – 2018-24), head group of the section „Sensors and Sensor systems” of the Dechema association (from 2024) or general secretary (2013-21) and president (2022-26) of the Bioelectrochemical Society (BES). Furthermore, he is active in the European Biosensor Symposiums conference series. 2024 he was awarded with the Bioelectrochemistry Prize of the International Society of Electrochemistry
ABOUT RESEARCH
Sensing systems based on photoelectrochemistry exploiting semiconductor nanostructures on electrodes – principles, bottlenecks and advancements –
Fred Lisdat
Biosystems Technology, Institute of Life Sciences and Biomedical Technologies, Technical University of Applied Sciences Wildau, 15745 Wildau, Germany
flisdat@th-wildau.de
Photoelectrochemical detection systems have gained interest in the analytical field since they allow a spatially resolved read-out by light illumination [1]. Operation is based on charge carrier generation within the semiconductor nanostructures by excitation and coupled electron transfer reactions [2,3]. This kind of transduction ensures good sensitivity, allows a detailed analysis of the sensing surface and a multiplexing of the detection. Semiconductor nanoparticles (quantum dots QDs) are often applied because of rather simple synthesis strategies, proper surface modification protocols and easy immobilization. In addition, they can be combined with very specific enzyme reactions. However, limitations may occur in practical applications, mainly due to rather small sensor signals, drift in the output and also for systems for which a close contact of the sensing surface with biological entities is essential (e.g. cell cultures).
These bottlenecks have been addressed by different approaches [4-6]. In a recent work a second semiconductor has been applied on top of the QD electrode – TiO2. This high band gap material is not excited under visible light conditions, but it supports charge carrier separation within the QDs. This new approach has been combined with a layer-by-layer deposition of the QDs on the electrode surface as previously introduced [7,8]. The resulting electrode system shows significantly increased current signals upon illumination, an enhanced signal to noise ratio, a very small drift in the sensor output and in addition, an improved sensing performance with respect to hydrogen peroxide which can be converted at the illuminated system [9]. It is further demonstrated that cells can be cultivated on top of the electrode structure without impacting the cell viability – exemplified with HeLa cells. After stimulation the release of hydrogen peroxide could successfully be verified.
By these investigations it can be demonstrated that the advantages of photoelectrochemical sensing systems provided by the light-directed read-out can be combined with an improved analytical performance such as enhanced sensitivity and stability – and therefore, resulting in improved applicability.
References
[1] Yue, Z.; Lisdat, F.; Parak, W. J.; Hickey, S. G.; Tu, L.; Sabir, N.; Dorfs, D.; Bigall, N. C. ACS Appl. Mater. Interfaces 5 (2013) 2800−2814.
[2] M. Riedel, W.J. Parak, A. Ruff, W. Schuhmann, F. Lisdat, ACS Catalysis 8 (2018) 5212
[3] S. Zhao, J. Völkner, M. Riedel, G. Witte, Z. Yue, F. Lisdat, W. J. Parak, ACS Applied Materials & Interfaces, 11 (24), (2019) 21830-21839
[4] M. Riedel, S. Hölzel, P. Hille, J. Schörmann, M. Eickhoff and F. Lisdat, Biosensors & Bioelectronics, (2017) Vol. 94, p. 298-304
[5] M. Riedel, F. Lisdat, ACS Applied Materials & Interfaces, 2018, Vol. 10 (1), p. 267-277
[6] S. Zhao, M. Riedel, J. Patarroyo, N. Bastus, V. Puntes, Z. Yue, F. Lisdat, W. J. Parak, Nanoscale 13 (2021) 980-990
[7] G. Göbel, K. Schubert, I. W. Schubart, W. Khalid, W. J. Parak, F. Lisdat, Electrochimica Acta 56 (18) (2011) 6397-6400
[8] S. Zhao, M. Riedel, J. Patarroyo, N. Bastús, V. Puntes, Y. Zhao, F, Lisdat, W. Parak, Nanoscale 14 (2022) 12048
[9] S. Zhao, Z. Yue, D. Zhu, J. Harberts, R. H. Blick, R. Zierold, F. Lisdat, W. J. Parak, Small, (2024), 2401703