1. Exploring the Alzheimer disease biomarkers: fabrication of new functional biomaterials and development of early diagnosis biosensor
Project Type: PCE, Start Date: 2021-01-04 End Date: 2023-12-28
2. On-line measurement of laser-driven proton beams effect on human cells
Project Type: ELI, Start Date: 2017-10-18 End Date: 2019-12-31
1. Novel cells integrated biosensor based on superoxide dismutase on electrospun fiber scaffolds for the electrochemical screening of cellular stress
Authors: Sanz, CG; Aldea, A; Oprea, D; Onea, M; Enache, AT; Barsan, MM
Published: JAN 15 2023, BIOSENSORS & BIOELECTRONICS, 220, 114858, DOI: 10.1016/j.bios.2022.114858
A novel electrochemical biosensor was developed to monitor fibroblast cells stress levels for the first time in situ under external stimuli based on the recognition of superoxide anion released upon cell damage. The biosensor comprised metallized polycaprolactone electrospun fibers covered with zinc oxide for improved cell adhesion and signal transduction, whilst stable bioconjugates of mercaptobenzoic acid-functionalized gold nanoparticles/ superoxide dismutase were employed as recognition bioelements. Biosensors were first tested and optimized for in situ generated superoxide detection by fixed potential amperometry at +0.3 V, with minimal interferences from electroactive species in cell culture media. L929 fibroblast cells were then implanted on the optimized biosensor surface and the biosensor morphologically characterized by scanning electron microscopy (SEM) and fluorescence microscopy, which illustrated the network-type pattern of fibroblasts adjacent to the fiber scaffold. Fibroblast stress was induced by zymosan and monitored at the cells integrated biosensor using fixed potential amperometry (CA) with a sensitivity of 26 nA cm-2 mu g mL-1 zymosan and electrochemical impedance spectros-copy (EIS), with similar sensitivity of the biosensor considering the Rs and Z' parameters of around 0.13 omega cm2 mu g-1 mL and high correlation factors R2 of 0.9994. The obtained results underline the applicability of the here developed biosensor for the electrochemical screening of the fibroblast cells stress. The concept in using low-cost biocompatible polymeric fibers as versatile scaffolds for both enzyme immobilization and cell adhesion, opens a new path in developing biosensors for the in-situ investigation of a variety of cellular events.
2. Nitrogen Functionalization of CVD Grown Three-Dimensional Graphene Foam for Hydrogen Evolution Reactions in Alkaline Media
Authors: Ion-Ebrasu, D; Andrei, RD; Enache, S; Caprarescu, S; Negrila, CC; Jianu, C; Enache, A; Boerasu, I; Carcadea, E; Varlam, M; Vasile, BS; Ren, JW
Published: SEP 2021, MATERIALS, 14, DOI: 10.3390/ma14174952
Three-dimensional graphene foam (3D-GrFoam) is a highly porous structure and sustained lattice formed by graphene layers with sp(2) and sp(3) hybridized carbon. In this work, chemical vapor deposition (CVD)-grown 3D-GrFoam was nitrogen-doped and platinum functionalized using hydrothermal treatment with different reducing agents (i.e., urea, hydrazine, ammonia, and dihydrogen hexachloroplatinate (IV) hydrate, respectively). X-ray photoelectron spectroscopy (XPS) survey showed that the most electrochemically active nitrogen-doped sample (GrFoam3N) contained 1.8 at % of N, and it exhibited a 172 mV dec(-1) Tafel plot associated with the Volmer-Heyrovsky hydrogen evolution (HER) mechanism in 0.1 M KOH. By the hydrothermal process, 0.2 at % of platinum was anchored to the graphene foam surface, and the resultant sample of GrFoamPt yielded a value of 80 mV dec(-1) Tafel associated with the Volmer-Tafel HER mechanism. Furthermore, Raman and infrared spectroscopy analysis, as well as scanning electron microscopy (SEM) were carried out to understand the structure of the samples.
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